Oligonucleotides

Dedicated Analytical Strategies

The term oligonucleotides includes a broad range of molecules with different chemical composition and covering a broad range of molecular weights, ranging between 15-20 nucleotides (e.g. siRNA) and many thousands of nucleotides (e.g. mRNA and DNA expressing whole genes); additionally, nucleic acid-based API are commonly administered with a suitable delivery vehicle protecting them from the environment and enhancing or targeting their delivery to the cell type where their action is required; lipid nanoparticles (LNPs) and virus like particles (VLPs) are common delivery methods. The recent introduction of novel genome editing technology such as CRISPR/Cas9 has further expanded the complexity of the nucleic acid repertoire, and innovative gene therapy platforms posing new analytical challenges are likely to emerge. With this complexity in mind and leveraging our experience in all the platforms described, Pace Life Sciences Boston, MA develops dedicated analytical strategies for each specific project.